4qtg

X-ray diffraction
1.47Å resolution

CRYSTAL STRUCTURE of 5-CARBOXYVANILLATE DECARBOXYLASE LIGW2 (TARGET EFI-505250) FROM NOVOSPHINGOBIUM AROMATICIVORANS DSM 12444 COMPLEXED WITH MANGANESE

Released:
Entry authors: Patskovsky Y, Vladimirova A, Toro R, Bhosle R, Gerlt JA, Raushel FM, Almo SC

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydro-rel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.46 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GA79 (Residues: 1-351; Coverage: 100%)
Gene name: Saro_0799
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 60.468Å b: 85.486Å c: 141.623Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.12 0.164
Expression system: Escherichia coli BL21(DE3)