› 4qt0

X-ray diffraction
3.2Å resolution

Crystal structure of human muscle L-lactate dehydrogenase in complex with inhibitor 1, 3-{[3-CARBAMOYL-7-(2,4-DIMETHOXYPYRIMIDIN-5-YL)QUINOLIN-4-YL]AMINO}BENZOIC ACID

Released:
DOI: 10.2210/pdb4qt0/pdb
PDB 4qt0 coloured by chain and viewed from the front.
PDB 4qt0 coloured by chain and viewed from the side.
PDB 4qt0 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structures of lactate dehydrogenase A (LDHA) in apo, ternary and inhibitor-bound forms.
Kolappan S, Shen DL, Mosi R, Sun J, McEachern EJ, Vocadlo DJ, Craig L
Acta Crystallogr. D Biol. Crystallogr. 71 185-95 (2015)
PMID: 25664730

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 337 amino acids
Theoretical weight: 37.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 38Q 8 x 38Q
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3121
Unit cell:
a: 147.19Å b: 147.19Å c: 334.63Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.172 0.271
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The Crosstalk Between Signaling Pathways and Cancer Metabolism in Colorectal Cancer.
Hon et al. (2021)
3 more

3 mentions without citation

Small Molecule-Based Enzyme Inhibitors in the Treatment of Primary Hyperoxalurias.
Moya-Garzon et al. (2021)
2 more