4qro

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE of DIHYDROXYBENZOIC ACID DECARBBOXYLASE BPRO_2061 (TARGET EFI-500288) FROM POLAROMONAS SP. JS666 WITH BOUND MANGANESE AND AN INHIBITOR, 2-NITRORESORCINOL

Released:
Source organism: Polaromonas sp. JS666
Entry authors: Patskovsky Y, Vladimirova A, Toro R, Bhosle R, Gerlt JA, Raushel M, Almo SC

Function and Biology Details

Reaction catalysed:
2,6-dihydroxybenzoate = 1,3-dihydroxybenzene + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-resorcylate decarboxylase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 348 amino acids
Theoretical weight: 40.21 KDa
Source organism: Polaromonas sp. JS666
Expression system: Not provided
UniProt:
  • Canonical: Q12BV1 (Residues: 1-326; Coverage: 100%)
Gene name: Bpro_2061
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 80.958Å b: 151.058Å c: 143.826Å
α: 90° β: 92.15° γ: 90°
R-values:
R R work R free
0.19 0.189 0.223
Expression system: Not provided