› 4qpm

X-ray diffraction
2.2Å resolution

Structure of Bub1 kinase domain

Released:
DOI: 10.2210/pdb4qpm/pdb
PDB entry 4qpm coloured by chain, front view.
PDB entry 4qpm coloured by chain, side view.
PDB entry 4qpm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substrate-specific activation of the mitotic kinase Bub1 through intramolecular autophosphorylation and kinetochore targeting.
Lin Z, Jia L, Tomchick DR, Luo X, Yu H
Structure 22 1616-27 (2014)
PMID: 25308863

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic checkpoint serine/threonine-protein kinase BUB1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 41.13 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43683 (Residues: 740-1085; Coverage: 32%)
Gene names: BUB1, BUB1L
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 4 x MG
Ligand CL 3 x CL
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 90.013Å b: 47.18Å c: 93.146Å
α: 90° β: 107.4° γ: 90°
R-values:
R R work R free
0.231 0.229 0.268
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Mechanisms of Mitotic Kinase Regulation: A Structural Perspective.
Welburn et al. (2018)
18 other articles

3 mentions without citation

Role of Intrinsic and Extrinsic Factors in the Regulation of the Mitotic Checkpoint Kinase Bub1.
Breit et al. (2015)
2 more