› 4qnz

X-ray diffraction
2.55Å resolution

Crystal structure of rhomboid intramembrane protease GlpG F146I in complex with peptide derived inhibitor Ac-FATA-cmk

Released:
DOI: 10.2210/pdb4qnz/pdb
PDB 4qnz coloured by chain and viewed from the front.
PDB 4qnz coloured by chain and viewed from the side.
PDB 4qnz coloured by chain and viewed from the top.
Source organism: Escherichia coli str. K-12 substr. MC4100
Primary publication:
Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
Zoll S, Stanchev S, Began J, Skerle J, Lepšík M, Peclinovská L, Majer P, Strisovsky K
EMBO J. (2014)
PMID: 25216680

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rhomboid protease GlpG Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.7 KDa
Source organism: Escherichia coli str. K-12 substr. MC4100
Expression system: Escherichia coli
Structure domains: Rhomboid-like
ACE-PHE-ALA-THR-ALA-0QE Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 467 Da
Source organism: Escherichia coli str. K-12 substr. MC4100
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand BNG 1 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P63
Unit cell:
a: 98.4Å b: 98.4Å c: 65.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.224
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Substrate-Enzyme Interactions in Intramembrane Proteolysis: γ-Secretase as the Prototype.
Liu et al. (2020)
6 more

1 mention without citation

Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
Zoll et al. (2014)