X-ray diffraction
1.8Å resolution

Crystal structure of EasA, an old yellow enzyme from Aspergillus fumigatus

Source organism: Aspergillus fumigatus Af293
Primary publication:
Structure of an Aspergillus fumigatus old yellow enzyme (EasA) involved in ergot alkaloid biosynthesis.
Acta Crystallogr F Struct Biol Commun 70 1328-32 (2014)
PMID: 25286934

Function and Biology Details

Reaction catalysed:
Dihydrochanoclavine-I aldehyde + NADP(+) = chanoclavine-I aldehyde + NADPH
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Chanoclavine-I aldehyde reductase easA Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.98 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q4WZ70 (Residues: 1-376; Coverage: 100%)
Gene names: AFUA_2G17960, easA, fgaOx3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 62.456Å b: 65.913Å c: 115.219Å
α: 90° β: 90° γ: 90°
R R work R free
0.182 0.178 0.221
Expression system: Escherichia coli BL21(DE3)