X-ray diffraction
2.8Å resolution

Crystal structure of the 2009 pandemic H1N1 influenza virus neuraminidase with a neutralizing antibody


Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 42.48 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
  • Canonical: D5KL82 (Residues: 82-467; Coverage: 82%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase
Immunoglobulin kappa constant Chains: F, L
Molecule details ›
Chains: F, L
Length: 212 amino acids
Theoretical weight: 23.27 KDa
Source organism: Mus musculus
Expression system: Not provided
  • Canonical: P01837 (Residues: 1-106; Coverage: 99%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
neutralizing antibody, heavy chain Chains: E, H
Molecule details ›
Chains: E, H
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C222
Unit cell:
a: 144.29Å b: 202.951Å c: 143.968Å
α: 90° β: 90° γ: 90°
R R work R free
0.19 0.181 0.224
Expression systems:
  • Trichoplusia ni
  • Not provided