› 4qmk

X-ray diffraction
2.5Å resolution

Crystal structure of type III effector protein ExoU (exoU)

Released:
DOI: 10.2210/pdb4qmk/pdb
PDB 4qmk coloured by chain and viewed from the front.
PDB 4qmk coloured by chain and viewed from the side.
PDB 4qmk coloured by chain and viewed from the top.
Source organism: Pseudomonas fluorescens A506
Primary publication:
A novel phosphatidylinositol 4,5-bisphosphate binding domain mediates plasma membrane localization of ExoU and other patatin-like phospholipases.
Tyson GH, Halavaty AS, Kim H, Geissler B, Agard M, Satchell KJ, Cho W, Anderson WF, Hauser AR
J Biol Chem 290 2919-37 (2015)
PMID: 25505182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type III secretion system effector protein ExoU Chains: A, B
Molecule details ›
Chains: A, B
Length: 677 amino acids
Theoretical weight: 72.77 KDa
Source organism: Pseudomonas fluorescens A506
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 67.218Å b: 115.341Å c: 88.443Å
α: 90° β: 102.77° γ: 90°
R-values:
R R work R free
0.209 0.206 0.27
Expression system: Escherichia coli

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Citations

3 review citations

Pseudomonas aeruginosa Toxin ExoU as a Therapeutic Target in the Treatment of Bacterial Infections.
Foulkes et al. (2019)
2 more

4 mentions without citation

A novel phosphatidylinositol 4,5-bisphosphate binding domain mediates plasma membrane localization of ExoU and other patatin-like phospholipases.
Tyson et al. (2015)
3 more