4qm1

X-ray diffraction
2.8Å resolution

Crystal Structure of the Inosine 5'-monophosphate Dehydrogenase with an Internal Deletion of the CBS Domain from Bacillus anthracis str. Ames complexed with inhibitor D67

Released:
Source organism: Bacillus anthracis
Entry authors: Kim Y, Makowska-Grzyska M, Gu M, Mandapati K, Gollapalli D, Gorla SK, Zhang M, Hedstrom L, Anderson WF, Joachimiak A, CSGID, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 384 amino acids
Theoretical weight: 40.71 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8P2U9 (Residues: 1-91, 221-487; Coverage: 74%)
Gene names: GBAA_0008, guaB
Sequence domains: IMP dehydrogenase / GMP reductase domain
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 83.13Å b: 101.331Å c: 87.267Å
α: 90° β: 109.57° γ: 90°
R-values:
R R work R free
0.195 0.192 0.248
Expression system: Escherichia coli