X-ray diffraction
1.45Å resolution

A novel phospho-switch in the linker region of the snail zinc finger protein which regulates 14-3-3 association, DNA binding and epithelial-mesenchymal differentiation

Source organism: Homo sapiens
Entry authors: Bier D, Ottmann C

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Zinc finger protein SNAI1 Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 738 Da
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: O95863 (Residues: 175-180; Coverage: 2%)
Gene names: SNAH, SNAI1

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 82.3Å b: 111.9Å c: 62.4Å
α: 90° β: 90° γ: 90°
R R work R free
0.171 0.169 0.203
Expression systems:
  • Escherichia coli
  • Not provided