› 4qko

X-ray diffraction
1.8Å resolution

The Crystal Structure of the Pyocin S2 Nuclease Domain, Immunity Protein Complex at 1.8 Angstroms

Released:
DOI: 10.2210/pdb4qko/pdb
PDB entry 4qko coloured by chain, front view.
PDB entry 4qko coloured by chain, side view.
PDB entry 4qko coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Grinter R, Josts I, Roszak AW, Cogdell CJ, Walker D

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170366 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pyocin-S2 immunity protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 95 amino acids
Theoretical weight: 11.13 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06579 (Residues: 1-87; Coverage: 100%)
Gene names: PA1151, imm2
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Pyocin-S2 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 134 amino acids
Theoretical weight: 14.52 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06584 (Residues: 556-689; Coverage: 19%)
Gene names: PA1150, pys2
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
Ligand BR 35 x BR
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 65.39Å b: 114.42Å c: 120.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.215
Expression system: Escherichia coli

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