4qjv

X-ray diffraction
1.6Å resolution

The X-ray crystal structure of Rpo3/Rpo11 heterodimer of euryarchaeal RNA polymerase from Thermococcus kodakarensis

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit Rpo3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 259 amino acids
Theoretical weight: 29.43 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JJF4 (Residues: 1-259; Coverage: 100%)
Gene names: TK1503, rpo3, rpoD
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit Rpo11 Chains: B, D
Molecule details ›
Chains: B, D
Length: 94 amino acids
Theoretical weight: 11.01 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JE88 (Residues: 1-94; Coverage: 100%)
Gene names: TK1167, rpo11, rpoL
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
Structure domains: RNA polymerase, RBP11-like subunit

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 76.598Å b: 88.169Å c: 102.907Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.211
Expression system: Escherichia coli