PDBe 4qjf

X-ray diffraction
2.31Å resolution

X-ray crystal structure of Thermocuccus kodakarensis RNA polymerase Rpp4/Rpo7 (RpoF/RpoE) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S1 motif domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.89 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JIY4 (Residues: 1-190; Coverage: 100%)
Gene name: TK1699
Sequence domains:
Structure domains:
DNA-directed RNA polymerase, subunit F Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 14.52 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JI52 (Residues: 1-114; Coverage: 99%)
Gene name: TK0901
Sequence domains: RNA polymerase Rpb4
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 42.465Å b: 80.942Å c: 97.413Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.261
Expression system: Escherichia coli