4qhs

X-ray diffraction
2.3Å resolution

Crystal structure of AAA+sigma 54 activator domain of the flagellar regulatory protein FlrC of Vibrio cholerae in nucleotide free state

Released:
Source organism: Vibrio cholerae O395
Entry authors: Dey S, Biswas M, Sen U, Dasgupta J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar regulatory protein C Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 267 amino acids
Theoretical weight: 29.83 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3AHP1 (Residues: 132-381; Coverage: 52%)
Gene names: VC0395_A1719, flrC
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 81.292Å b: 153.285Å c: 193.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.255
Expression system: Escherichia coli BL21(DE3)