› 4qh0

X-ray diffraction
2Å resolution

Crystal structure of NucA from Streptococcus agalactiae with magnesium ion bound

Released:
DOI: 10.2210/pdb4qh0/pdb
PDB 4qh0 coloured by chain and viewed from the front.
PDB 4qh0 coloured by chain and viewed from the side.
PDB 4qh0 coloured by chain and viewed from the top.
Source organism: Streptococcus agalactiae ILRI112
Primary publication:
Structural characterization of the virulence factor nuclease A from Streptococcus agalactiae.
Moon AF, Gaudu P, Pedersen LC
OpenAccess logo Acta Crystallogr. D Biol. Crystallogr. 70 2937-49 (2014)
PMID: 25372684

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-entry nuclease (Competence-specific nuclease) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.24 KDa
Source organism: Streptococcus agalactiae ILRI112
Expression system: Escherichia coli
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand SO4 23 x SO4
Ligand CL 6 x CL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P63
Unit cell:
a: 123.721Å b: 123.721Å c: 157.403Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.155 0.19
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Group B Streptococcus Induces Neutrophil Recruitment to Gestational Tissues and Elaboration of Extracellular Traps and Nutritional Immunity.
Kothary et al. (2017)
1 more

3 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
2 more