› 4qgv

X-ray diffraction
1.73Å resolution

Crystal structure of the R132K:R111L mutant of Cellular Retinoic Acid Binding ProteinII complexed with a synthetic ligand (Merocyanine) at 1.73 angstrom resolution.

Released:
DOI: 10.2210/pdb4qgv/pdb
PDB 4qgv coloured by chain and viewed from the front.
PDB 4qgv coloured by chain and viewed from the side.
PDB 4qgv coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
"Turn-On" Protein Fluorescence: In Situ Formation of Cyanine Dyes.
Yapici I, Lee KS, Berbasova T, Nosrati M, Jia X, Vasileiou C, Wang W, Santos EM, Geiger JH, Borhan B
J. Am. Chem. Soc. (2015)
PMID: 25534273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand LMC 1 x LMC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 34.785Å b: 37.34Å c: 60.091Å
α: 104.53° β: 106.04° γ: 90.87°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.
Cambré et al. (2020)
8 more

1 mention without citation

"Turn-on" protein fluorescence: in situ formation of cyanine dyes.
Yapici et al. (2015)