X-ray diffraction
1.5Å resolution

Crystal structure of NucA from Streptococcus agalactiae with no metal bound

Primary publication:
Structural characterization of the virulence factor nuclease A from Streptococcus agalactiae.
OpenAccess logo Acta Crystallogr. D Biol. Crystallogr. 70 2937-49 (2014)
PMID: 25372684

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA-entry nuclease (Competence-specific nuclease) Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.24 KDa
Source organism: Streptococcus agalactiae ILRI112
Expression system: Escherichia coli
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 37.591Å b: 57.131Å c: 62.422Å
α: 109.4° β: 97.08° γ: 90.16°
R R work R free
0.154 0.153 0.18
Expression system: Escherichia coli