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Joint X-ray and neutron structure of Streptomyces rubiginosus D-xylose isomerase in complex with two Cd2+ ions and cyclic beta-L-arabinose

Neutron Diffraction
PDB entry 4qdp coloured by chain, front view.
PDB entry 4qdp coloured by chain, side view.
PDB entry 4qdp coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Xylose isomerase
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Multimeric state: homo tetramer
Accessible surface area: 46313.28 Å2
Buried surface area: 34297.89 Å2
Dissociation area: 12,892.88 Å2
Dissociation energy (ΔGdiss): 218.12 kcal/mol
Dissociation entropy (TΔSdiss): 18.4 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-150204

Macromolecules

Xylose isomerase
Chain: A
Length: 388 amino acids
Theoretical weight: 43.28 KDa
Source organism: Streptomyces rubiginosus
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Pfam: Xylose isomerase-like TIM barrel
InterPro:
CATH: Divalent-metal-dependent TIM barrel enzymes
Xylose isomerase in PDB entry 4qdp, assembly 1, front view.
Xylose isomerase in PDB entry 4qdp, assembly 1, side view.
Xylose isomerase in PDB entry 4qdp, assembly 1, top view.
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