› 4qdk

X-ray diffraction
1.7Å resolution

Crystal structure of magnesium protoporphyrin IX methyltransferase (ChlM) from Synechocystis PCC 6803 with bound SAH

Released:
DOI: 10.2210/pdb4qdk/pdb
PDB 4qdk coloured by chain and viewed from the front.
PDB 4qdk coloured by chain and viewed from the side.
PDB 4qdk coloured by chain and viewed from the top.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Structural insights into the catalytic mechanism of Synechocystis magnesium protoporphyrin IX O-methyltransferase (ChlM).
Chen X, Wang X, Feng J, Chen Y, Fang Y, Zhao S, Zhao A, Zhang M, Liu L
J. Biol. Chem. 289 25690-8 (2014)
PMID: 25077963

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + magnesium protoporphyrin IX = S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Magnesium-protoporphyrin O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.18 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55467 (Residues: 1-230; Coverage: 100%)
Gene names: chlM, slr0525
Sequence domains: Magnesium-protoporphyrin IX methyltransferase C-terminus
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 49.059Å b: 98.876Å c: 52.935Å
α: 90° β: 95.84° γ: 90°
R-values:
R R work R free
0.19 0.188 0.224
Expression system: Escherichia coli

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Citations

3 review citations

The terminal enzymes of (bacterio)chlorophyll biosynthesis.
Proctor et al. (2022)
2 more

1 mention without citation

Structural insights into the catalytic mechanism of Synechocystis magnesium protoporphyrin IX O-methyltransferase (ChlM).
Chen et al. (2014)