4qbk

X-ray diffraction
1.77Å resolution

Crystal structure of the complex of Peptidyl-tRNA hydrolase from Pseudomonas aeruginosa with amino acyl-tRNA analogue at 1.77 Angstrom resolution

Released:
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Singh A, Sinha M, Bhushan A, Kaur P, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed:
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 20.83 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVC3 (Residues: 1-194; Coverage: 100%)
Gene names: PA4672, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6122
Unit cell:
a: 64.751Å b: 64.751Å c: 157.679Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.183
Expression system: Escherichia coli