› 4qak

X-ray diffraction
2.02Å resolution

Crystal structure of phosphoesterase

Released:
DOI: 10.2210/pdb4qak/pdb
PDB 4qak coloured by chain and viewed from the front.
PDB 4qak coloured by chain and viewed from the side.
PDB 4qak coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Entry authors: Remus BS, Shuman S

Function and Biology Details

Reaction catalysed: 
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA 2',3'-cyclic phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.19 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P37025 (Residues: 1-176; Coverage: 100%)
Gene names: JW5011, b0147, ligT, thpR, yadP
Sequence domains: LigT like Phosphoesterase
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand 2AM 1 x 2AM
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4212
Unit cell:
a: 107.688Å b: 107.688Å c: 71.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.229

Quick links