X-ray diffraction
2.02Å resolution

Crystal structure of phosphoesterase

Source organism: Escherichia coli K-12
Entry authors: Remus BS, Shuman S

Function and Biology Details

Reaction catalysed:
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
RNA 2',3'-cyclic phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.19 KDa
Source organism: Escherichia coli K-12
  • Canonical: P37025 (Residues: 1-176; Coverage: 100%)
Gene names: JW5011, b0147, ligT, thpR, yadP
Sequence domains: LigT like Phosphoesterase
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4212
Unit cell:
a: 107.688Å b: 107.688Å c: 71.657Å
α: 90° β: 90° γ: 90°
R R work R free
0.177 0.174 0.229