4qa3

X-ray diffraction
2.88Å resolution

Crystal structure of T311M HDAC8 in complex with Trichostatin A (TSA)

Released:
Source organism: Homo sapiens
Entry authors: Decroos C, Bowman CB, Moser J-AS, Christianson KE, Deardorff MA, Christianson DW

Function and Biology Details

Reaction catalysed:
Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone deacetylase 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BY41 (Residues: 1-377; Coverage: 100%)
Gene names: CDA07, HDAC8, HDACL1
Sequence domains: Histone deacetylase domain
Structure domains: Histone deacetylase domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 51.186Å b: 83.094Å c: 94.297Å
α: 90° β: 95.94° γ: 90°
R-values:
R R work R free
0.183 0.178 0.234
Expression system: Escherichia coli BL21(DE3)