› 4q9n

X-ray diffraction
1.8Å resolution

Crystal structure of Chlamydia trachomatis enoyl-ACP reductase (FabI) in complex with NADH and AFN-1252

Released:
DOI: 10.2210/pdb4q9n/pdb
PDB 4q9n coloured by chain and viewed from the front.
PDB 4q9n coloured by chain and viewed from the side.
PDB 4q9n coloured by chain and viewed from the top.
Source organism: Chlamydia trachomatis F/11-96
Primary publication:
Type II Fatty Acid Synthesis Is Essential for the Replication of Chlamydia trachomatis.
Yao J, Abdelrahman YM, Robertson RM, Cox JV, Belland RJ, White SW, Rock CO
J. Biol. Chem. 289 22365-76 (2014)
PMID: 24958721

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 298 amino acids
Theoretical weight: 31.99 KDa
Source organism: Chlamydia trachomatis F/11-96
Expression system: Escherichia coli BL21(DE3)
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAI 8 x NAI
Ligand 0WE 8 x 0WE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43
Unit cell:
a: 96.146Å b: 96.146Å c: 263.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Modulation of Host Lipid Pathways by Pathogenic Intracellular Bacteria.
Allen et al. (2020)
6 more

4 mentions without citation

Type II fatty acid synthesis is essential for the replication of Chlamydia trachomatis.
Yao et al. (2014)
3 more