› 4q60

X-ray diffraction
2.1Å resolution

Crystal structure of a 4-hydroxyproline epimerase from Burkholderia Multivorans atcc 17616, target EFI-506586, open form, with bound pyrrole-2-carboxylate

Released:
DOI: 10.2210/pdb4q60/pdb
PDB entry 4q60 coloured by chain, front view.
PDB entry 4q60 coloured by chain, side view.
PDB entry 4q60 coloured by chain, top view.
Source organism: Burkholderia multivorans ATCC 17616
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi N, Sojitra S, Stead M, Washington E, Glenn AS, Chowdhury S, Evans B, Hammonds J, Hillerich B, Love J, Seidel RD, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyproline 2-epimerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 35.36 KDa
Source organism: Burkholderia multivorans ATCC 17616
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3D6W2 (Residues: 2-311; Coverage: 100%)
Gene names: BMULJ_04062, Bmul_4447, prdF
Sequence domains: Proline racemase
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PYC 2 x PYC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 82.749Å b: 95.053Å c: 126.275Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links