PDBe 4q5h

X-ray diffraction
2Å resolution

Shigella Effector Kinase OspG bound to AMPPNP and E2-Ub UbcH7-Ub Conjugate

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein kinase OspG Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.12 KDa
Source organism: Shigella sonnei Ss046
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3YTH2 (Residues: 26-196; Coverage: 87%)
Gene names: SSON_P170, ospG
Structure domains:
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG63 (Residues: 305-380; Coverage: 20%)
Gene names: SCD2, UBI4, YLL039C
Sequence domains: Ubiquitin family
Ubiquitin-conjugating enzyme E2 L3 Chain: C
Molecule details ›
Chain: C
Length: 156 amino acids
Theoretical weight: 18.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68036 (Residues: 1-154; Coverage: 100%)
Gene names: UBCE7, UBCH7, UBE2L3
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 67.416Å b: 84.658Å c: 85.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.228
Expression system: Escherichia coli BL21(DE3)