X-ray diffraction
1.93Å resolution

Structure and mechanism of a dehydratase/decarboxylase enzyme couple involved in polyketide beta-branching

Entry authors: Nair AV, Race PR, Till M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Putative polyketide biosynthesis enoyl-CoA isomerase PksI Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 268 amino acids
Theoretical weight: 30.08 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
  • Canonical: P40802 (Residues: 1-249; Coverage: 100%)
Gene names: BSU17170, pksI
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 82.89Å b: 84.66Å c: 125.92Å
α: 90° β: 90° γ: 90°
R R work R free
0.151 0.149 0.185
Expression system: Escherichia coli