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The catalytic core of Rad2 in complex with DNA substrate (complex IV)

X-ray diffraction
2.7Å resolution
PDB entry 4q10 coloured by chain, front view.
PDB entry 4q10 coloured by chain, side view.
PDB entry 4q10 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA repair protein RAD2 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 33276.25 Å2
Buried surface area: 5114.45 Å2
Dissociation area: 2,366.44 Å2
Dissociation energy (ΔGdiss): 27.93 kcal/mol
Dissociation entropy (TΔSdiss): 35.43 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118002

Macromolecules

DNA repair protein RAD2
Chains: A, B
Length: 365 amino acids
Theoretical weight: 42.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07276 (Residues: 2-986; Coverage: 35%)
Gene names: RAD2, YGR258C
Pfam:
InterPro:
DNA repair protein RAD2 in PDB entry 4q10, assembly 1, front view.
DNA repair protein RAD2 in PDB entry 4q10, assembly 1, side view.
DNA repair protein RAD2 in PDB entry 4q10, assembly 1, top view.
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DNA chain C
Name: DNA (5'-D(*TP*TP*TP*TP*GP*CP*TP*CP*CP*CP*TP*TP*GP*TP*CP*TP*CP*AP*GP*TP*TP*TP*T)-3')
Representative chains: C
Source organism: Saccharomyces cerevisiae S288C [559292]
Expression system: Not provided
Length: 23 nucleotides
Theoretical weight: 6.95 KDa
DNA (5'-D(*TP*TP*TP*TP*GP*CP*TP*CP*CP*CP*TP*TP*GP*TP*CP*TP*CP*AP*GP*TP*TP*TP*T)-3') in PDB entry 4q10, assembly 1, front view.
DNA (5'-D(*TP*TP*TP*TP*GP*CP*TP*CP*CP*CP*TP*TP*GP*TP*CP*TP*CP*AP*GP*TP*TP*TP*T)-3') in PDB entry 4q10, assembly 1, side view.
DNA (5'-D(*TP*TP*TP*TP*GP*CP*TP*CP*CP*CP*TP*TP*GP*TP*CP*TP*CP*AP*GP*TP*TP*TP*T)-3') in PDB entry 4q10, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(*TP*TP*TP*TP*CP*TP*GP*AP*GP*AP*CP*AP*AP*GP*GP*GP*AP*GP*CP*TP*TP*TP*T)-3')
Representative chains: D
Source organism: Saccharomyces cerevisiae S288C [559292]
Expression system: Not provided
Length: 23 nucleotides
Theoretical weight: 7.1 KDa
DNA (5'-D(*TP*TP*TP*TP*CP*TP*GP*AP*GP*AP*CP*AP*AP*GP*GP*GP*AP*GP*CP*TP*TP*TP*T)-3') in PDB entry 4q10, assembly 1, front view.
DNA (5'-D(*TP*TP*TP*TP*CP*TP*GP*AP*GP*AP*CP*AP*AP*GP*GP*GP*AP*GP*CP*TP*TP*TP*T)-3') in PDB entry 4q10, assembly 1, side view.
DNA (5'-D(*TP*TP*TP*TP*CP*TP*GP*AP*GP*AP*CP*AP*AP*GP*GP*GP*AP*GP*CP*TP*TP*TP*T)-3') in PDB entry 4q10, assembly 1, top view.
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