4q10

X-ray diffraction
2.7Å resolution

The catalytic core of Rad2 in complex with DNA substrate (complex IV)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein RAD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 42.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07276 (Residues: 730-986; Coverage: 25%)
Gene names: RAD2, YGR258C
Sequence domains: XPG I-region
DNA (5'-D(*TP*TP*TP*TP*GP*CP*TP*CP*CP*CP*TP*TP*GP*TP*CP*TP*CP*AP*GP*TP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 6.95 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*TP*CP*TP*GP*AP*GP*AP*CP*AP*AP*GP*GP*GP*AP*GP*CP*TP*TP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.1 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 98.68Å b: 97.958Å c: 112.038Å
α: 90° β: 96.41° γ: 90°
R-values:
R R work R free
0.234 0.232 0.284
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided