4q0t

X-ray diffraction
1.7Å resolution

Free form of TvNiR, low dose data set

Released:
Entry authors: Lazarenko VA, Polyakov KM, Trofimov AA, Popov AN, Tikhonova TV, Tikhonov AV, Popov VO

Function and Biology Details

Reaction catalysed:
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c-552 Chains: A, B
Molecule details ›
Chains: A, B
Length: 524 amino acids
Theoretical weight: 59.36 KDa
Source organism: Thioalkalivibrio nitratireducens
UniProt:
  • Canonical: L0DSL2 (Residues: 30-553; Coverage: 100%)
Gene names: TVNIR_0259, nir
Sequence domains: Cytochrome c552
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 16 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P213
Unit cell:
a: 195.48Å b: 195.48Å c: 195.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.17