› 4pzy

X-ray diffraction
1.88Å resolution

Second-site screening of K-Ras in the presence of covalently attached first-site ligands

Released:
DOI: 10.2210/pdb4pzy/pdb
PDB entry 4pzy coloured by chain, front view.
PDB entry 4pzy coloured by chain, side view.
PDB entry 4pzy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A method for the second-site screening of K-Ras in the presence of a covalently attached first-site ligand.
Sun Q, Phan J, Friberg AR, Camper DV, Olejniczak ET, Fesik SW
J Biomol NMR 60 11-4 (2014)
PMID: 25087006

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand 2XR 2 x 2XR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 44.101Å b: 55.964Å c: 120.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.238
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Genetics and biology of pancreatic ductal adenocarcinoma.
Ying et al. (2016)
8 more

4 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
3 more