4pyh

X-ray diffraction
1.65Å resolution

Phospho-glucan bound structure of starch phosphatase Starch EXcess4 reveals the mechanism for C6-specificty

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Phosphoglucan phosphatase DSP4, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.51 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9FEB5 (Residues: 90-379; Coverage: 77%)
Gene names: At3g52180, DSP4, F4F15.290, PTPKIS1, SEX4
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 33.475Å b: 77.893Å c: 117.213Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.218
Expression system: Escherichia coli BL21