› 4pyh

X-ray diffraction
1.65Å resolution

Phospho-glucan bound structure of starch phosphatase Starch EXcess4 reveals the mechanism for C6-specificty

Released:
DOI: 10.2210/pdb4pyh/pdb
PDB 4pyh coloured by chain and viewed from the front.
PDB 4pyh coloured by chain and viewed from the side.
PDB 4pyh coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Primary publication:
Phosphoglucan-bound structure of starch phosphatase Starch Excess4 reveals the mechanism for C6 specificity.
Meekins DA, Raththagala M, Husodo S, White CJ, Guo HF, Kötting O, Vander Kooi CW, Gentry MS
Proc Natl Acad Sci U S A 111 7272-7 (2014)
PMID: 24799671

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Phosphoglucan phosphatase DSP4, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.51 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9FEB5 (Residues: 90-379; Coverage: 77%)
Gene names: At3g52180, DSP4, F4F15.290, PTPKIS1, SEX4
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 33.475Å b: 77.893Å c: 117.213Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Brain Glycogen Structure and Its Associated Proteins: Past, Present and Future.
Brewer et al. (2019)
5 more

6 mentions without citation

Functional Roles of Starch Binding Domains and Surface Binding Sites in Enzymes Involved in Starch Biosynthesis.
Wilkens et al. (2018)
5 more