4py8

X-ray diffraction
2.91Å resolution

Crystal structure of Fab 3.1 in complex with the 1918 influenza virus hemagglutinin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 36.45 KDa
Source organism: Influenza A virus (A/South Carolina/1/1918(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9WFX3 (Residues: 18-344; Coverage: 60%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 179 amino acids
Theoretical weight: 20.39 KDa
Source organism: Influenza A virus (A/South Carolina/1/1918(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9WFX3 (Residues: 345-520; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
antibody 3.1 heavy chain Chain: I
Molecule details ›
Chain: I
Length: 219 amino acids
Theoretical weight: 23.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody 3.1 light chain Chain: J
Molecule details ›
Chain: J
Length: 214 amino acids
Theoretical weight: 23.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: R3
Unit cell:
a: 135.06Å b: 135.06Å c: 230.201Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.242
Expression systems:
  • Trichoplusia ni
  • Escherichia coli