› 4pxt

X-ray diffraction
2.9Å resolution

Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers

Released:
DOI: 10.2210/pdb4pxt/pdb
PDB 4pxt coloured by chain and viewed from the front.
PDB 4pxt coloured by chain and viewed from the side.
PDB 4pxt coloured by chain and viewed from the top.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers.
Scholey JE, Nithianantham S, Scholey JM, Al-Bassam J
OpenAccess logo Elife 3 e02217 (2014)
PMID: 24714498

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein Klp61F Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 24.98 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P46863 (Residues: 634-839; Coverage: 19%)
Gene names: CG9191, KLP2, Klp61F

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P6422
Unit cell:
a: 139.07Å b: 139.07Å c: 104.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.242 0.274
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Bidirectional motility of kinesin-5 motor proteins: structural determinants, cumulative functions and physiological roles.
Singh et al. (2018)
4 more

1 mention without citation

Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers.
Scholey et al. (2014)