4pxt

X-ray diffraction
2.9Å resolution

Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein Klp61F Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 24.98 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P46863 (Residues: 634-839; Coverage: 19%)
Gene names: CG9191, KLP2, Klp61F

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P6422
Unit cell:
a: 139.07Å b: 139.07Å c: 104.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.242 0.274
Expression system: Escherichia coli BL21