› 4pur

X-ray diffraction
2.95Å resolution

Crystal structure of MglA from Francisella tularensis

Released:
DOI: 10.2210/pdb4pur/pdb
PDB entry 4pur coloured by chain, front view.
PDB entry 4pur coloured by chain, side view.
PDB entry 4pur coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Primary publication:
Structural and Biochemical Characterization of the Francisella tularensis Pathogenicity Regulator, Macrophage Locus Protein A (MglA).
Cuthbert BJ, Brennan RG, Schumacher MA
PLoS One 10 e0128225 (2015)
PMID: 26121147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GST N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 24.16 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NFG1 (Residues: 2-205; Coverage: 100%)
Gene names: FTT_1275, mglA
Sequence domains: Glutathione S-transferase, N-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MLT 2 x MLT
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P61
Unit cell:
a: 104.684Å b: 104.684Å c: 100.045Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.172 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Diverse molecular mechanisms of transcription regulation by the bacterial alarmone ppGpp.
Travis et al. (2022)
1 more

2 mentions without citation

Dissection of the molecular circuitry controlling virulence in Francisella tularensis.
Cuthbert et al. (2017)
1 more