› 4pu8

X-ray diffraction
2.35Å resolution

Shewanella oneidensis Toxin Antitoxin System Antitoxin Protein HipB Resolution 2.35

Released:
DOI: 10.2210/pdb4pu8/pdb
PDB entry 4pu8 coloured by chain, front view.
PDB entry 4pu8 coloured by chain, side view.
PDB entry 4pu8 coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Primary publication:
The bacterial antitoxin HipB establishes a ternary complex with operator DNA and phosphorylated toxin HipA to regulate bacterial persistence.
Wen Y, Behiels E, Felix J, Elegheert J, Vergauwen B, Devreese B, Savvides SN
Nucleic Acids Res 42 10134-47 (2014)
PMID: 25056321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antitoxin HipB Chains: A, B
Molecule details ›
Chains: A, B
Length: 118 amino acids
Theoretical weight: 12.95 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EIX4 (Residues: 1-78; Coverage: 100%)
Gene names: SO_0705, hipB
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2221
Unit cell:
a: 53.57Å b: 71.57Å c: 85.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.224
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Keeping the Wolves at Bay: Antitoxins of Prokaryotic Type II Toxin-Antitoxin Systems.
Chan et al. (2016)
4 more

2 mentions without citation

Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.
Coussens et al. (2016)
1 more