› 4ptm

X-ray diffraction
1.7Å resolution

Crystal Structure of Chitinase D from Serratia proteamaculans in complex with N-acetyl glucosamine, a hydrolyzed product of hexasaccharide at 1.7 Angstrom resolution

Released:
DOI: 10.2210/pdb4ptm/pdb
PDB entry 4ptm coloured by chain, front view.
PDB entry 4ptm coloured by chain, side view.
PDB entry 4ptm coloured by chain, top view.
Source organism: Serratia proteamaculans 568
Entry authors: Kushwaha GS, Madhuprakash J, Singh A, Bhushan A, Sinha M, Kaur P, Sharma S, Podile AR, Singh TP

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.51 KDa
Source organism: Serratia proteamaculans 568
Expression system: Escherichia coli
UniProt:
  • Canonical: A8GFD6 (Residues: 24-426; Coverage: 100%)
Gene name: Spro_2725
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 1 x ACT
Ligand NAG 1 x NAG
Ligand GOL 2 x GOL
1 modified residue:
Ligand OMT 1 x OMT

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Unit cell:
a: 59.418Å b: 74.064Å c: 88.745Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.193
Expression system: Escherichia coli

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