4pth

X-ray diffraction
0.85Å resolution

Ensemble model for Escherichia coli dihydrofolate reductase at 100K

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.05 KDa
Source organism: Escherichia coli str. K-12 substr. DH10B
Expression system: Escherichia coli BL21(DE3)
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 33.96Å b: 44.823Å c: 98.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 0.126 0.144
Expression system: Escherichia coli BL21(DE3)