Function and Biology Details
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
- not assigned
Structure analysis Details
Length: 324 amino acids
Theoretical weight: 36.04 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21
- Canonical: K4LC41 (Residues: 1482-1803; Coverage: 7%)