› 4pqg

X-ray diffraction
2Å resolution

Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc

Released:
DOI: 10.2210/pdb4pqg/pdb
PDB entry 4pqg coloured by chain, front view.
PDB entry 4pqg coloured by chain, side view.
PDB entry 4pqg coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structure of a novel O-linked N-acetyl-D-glucosamine (O-GlcNAc) transferase, GtfA, reveals insights into the glycosylation of pneumococcal serine-rich repeat adhesins.
Shi WW, Jiang YL, Zhu F, Yang YH, Shao QY, Yang HB, Ren YM, Wu H, Chen Y, Zhou CZ
J Biol Chem 289 20898-907 (2014)
PMID: 24936067

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-101469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 511 amino acids
Theoretical weight: 58.86 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2URG7 (Residues: 1-503; Coverage: 100%)
Gene names: SP_1758, gtfA
Sequence domains: Glycosyl transferases group 1
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand UDP 2 x UDP
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 71.24Å b: 125.63Å c: 127.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
10 more

4 mentions without citation

Structure of a novel O-linked N-acetyl-D-glucosamine (O-GlcNAc) transferase, GtfA, reveals insights into the glycosylation of pneumococcal serine-rich repeat adhesins.
Shi et al. (2014)
3 more