4pp8

X-ray diffraction
1.95Å resolution

Crystal structure of murine NK cell ligand RAE-1 beta in complex with NKG2D

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NKG2-D type II integral membrane protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O54709 (Residues: 109-232; Coverage: 53%)
Gene names: Klrk1, Nkg2d
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Retinoic acid early-inducible protein 1-beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 174 amino acids
Theoretical weight: 20.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08603 (Residues: 31-204; Coverage: 77%)
Gene name: Raet1b
Sequence domains: Class I Histocompatibility antigen, NKG2D ligand, domains 1 and 2
Structure domains: MHC class I-like antigen recognition-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P61
Unit cell:
a: 58.637Å b: 58.637Å c: 350.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.217
Expression system: Escherichia coli BL21(DE3)