› 4poy

X-ray diffraction
1.5Å resolution

Engineered Dual Specific VHH antibody

Released:
DOI: 10.2210/pdb4poy/pdb
PDB entry 4poy coloured by chain, front view.
PDB entry 4poy coloured by chain, side view.
PDB entry 4poy coloured by chain, top view.
Source organism: Lama glama
Primary publication:
Structural basis of an engineered dual-specific antibody: conformational diversity leads to a hypervariable loop metal-binding site.
Fanning SW, Walter R, Horn JR
Protein Eng Des Sel 27 391-7 (2014)
PMID: 25143596

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VHH-metal Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 12.94 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2221
Unit cell:
a: 53.753Å b: 61.979Å c: 64.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.241
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and development of single domain antibodies as modules for therapeutics and diagnostics.
Hoey et al. (2019)
3 other articles

2 mentions without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)
1 more