4pne

X-ray diffraction
1.5Å resolution

Crystal Structure of the [4+2]-Cyclase SpnF

Released:
Source organism: Saccharopolyspora spinosa
Entry authors: Fage CD, Isiorho EA, Liu Y-N, Liu H-W, Keatinge-Clay AT

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransf_11 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 32.62 KDa
Source organism: Saccharopolyspora spinosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ALM7 (Residues: 2-275; Coverage: 100%)
Gene name: spnF
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 41.74Å b: 69.35Å c: 83.63Å
α: 90° β: 97.49° γ: 90°
R-values:
R R work R free
0.198 0.197 0.22
Expression system: Escherichia coli BL21(DE3)