› 4pmr

X-ray diffraction
1.81Å resolution

Crystal structure of the Mycobacterium tuberculosis Tat-secreted protein Rv2525c in complex with HEPES (monoclinic crystal form II)

Released:
DOI: 10.2210/pdb4pmr/pdb
PDB entry 4pmr coloured by chain, front view.
PDB entry 4pmr coloured by chain, side view.
PDB entry 4pmr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Structural studies suggest a peptidoglycan hydrolase function for the Mycobacterium tuberculosis Tat-secreted protein Rv2525c.
Bellinzoni M, Haouz A, Miras I, Magnet S, André-Leroux G, Mukherjee R, Shepard W, Cole ST, Alzari PM
J Struct Biol 188 156-64 (2014)
PMID: 25260828

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rv2525c-like glycoside hydrolase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.04 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P95028 (Residues: 36-240; Coverage: 85%)
Gene name: MT2601
Sequence domains: Rv2525c-like, glycoside hydrolase-like domain
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand EPE 1 x EPE
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 81.98Å b: 62.5Å c: 38.73Å
α: 90° β: 109.84° γ: 90°
R-values:
R R work R free
0.171 0.169 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

The hydrolase LpqI primes mycobacterial peptidoglycan recycling.
Moynihan et al. (2019)
4 more