X-ray diffraction
2.2Å resolution

Crystal structure of a putative oxidoreductase from Sinorhizobium meliloti 1021 in complex with NADP

Source organism: Sinorhizobium meliloti 1021
Entry authors: Gasiorowska OA, Shabalin IG, Handing KB, Szlachta K, Zimmerman MD, Hillerich BS, Gizzi A, Toro R, Bonanno J, Seidel R, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Aldo_ket_red domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 31.09 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q92NR7 (Residues: 1-281; Coverage: 100%)
Gene name: SMc01429
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 44.324Å b: 78.897Å c: 79.563Å
α: 90° β: 90° γ: 90°
R R work R free
0.17 0.167 0.217
Expression system: Escherichia coli BL21(DE3)