4pir

X-ray diffraction
3.5Å resolution

X-ray structure of the mouse serotonin 5-HT3 receptor

Released:
Source organisms:
Entry authors: Hassaine G, Deluz C, Grasso L, Wyss R, Tol MB, Hovius R, Graff A, Stahlberg H, Tomizaki T, Desmyter A, Moreau C, Li X-D, Poitevin F, Vogel H, Nury H

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
5-hydroxytryptamine receptor 3A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 456 amino acids
Theoretical weight: 52.61 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P23979 (Residues: 28-31, 32-302, 304-487; Coverage: 98%)
  • Best match: P23979-2 (Residues: 28-31, 32-302, 304-481)
Gene names: 5ht3, Htr3, Htr3a
Sequence domains:
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
VHH15 Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 124 amino acids
Theoretical weight: 13.46 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 121.542Å b: 187.087Å c: 240.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.257
Expression systems:
  • Homo sapiens
  • Escherichia coli