› 4pir

X-ray diffraction
3.5Å resolution

X-ray structure of the mouse serotonin 5-HT3 receptor

Released:
DOI: 10.2210/pdb4pir/pdb
PDB 4pir coloured by chain and viewed from the front.
PDB 4pir coloured by chain and viewed from the side.
PDB 4pir coloured by chain and viewed from the top.
Source organisms:
Entry authors: Hassaine G, Deluz C, Grasso L, Wyss R, Tol MB, Hovius R, Graff A, Stahlberg H, Tomizaki T, Desmyter A, Moreau C, Li X-D, Poitevin F, Vogel H, Nury H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
5-hydroxytryptamine receptor 3A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 456 amino acids
Theoretical weight: 52.61 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P23979 (Residues: 28-31, 32-302, 304-487; Coverage: 98%)
  • Best match: P23979-2 (Residues: 28-31, 32-302, 304-481)
Gene names: 5ht3, Htr3, Htr3a
Sequence domains:
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
VHH15 Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 124 amino acids
Theoretical weight: 13.46 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand CL 5 x CL
Ligand SO4 3 x SO4
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 121.542Å b: 187.087Å c: 240.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.257
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

51 review citations

An Inside Job: Molecular Determinants for Postsynaptic Localization of Nicotinic Acetylcholine Receptors.
Ferns M. (2021)
50 more

28 mentions without citation

Understanding structure-function relationships of the human neuronal acetylcholine receptor: insights from the first crystal structures of neuronal subunits.
Giastas et al. (2018)
27 more