› 4pfa

X-ray diffraction
2.56Å resolution

Crystal structure of M. tuberculosis in complex with BTO - covalent adduct

Released:
DOI: 10.2210/pdb4pfa/pdb
PDB 4pfa coloured by chain and viewed from the front.
PDB 4pfa coloured by chain and viewed from the side.
PDB 4pfa coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Neres J, Panda M, Cole S

Function and Biology Details

Reaction catalysed: 
Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decaprenylphosphoryl-beta-D-ribose oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 52.26 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJF1 (Residues: 1-461; Coverage: 100%)
Gene names: Rv3790, dprE1
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand N77 2 x N77
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 72.856Å b: 82.013Å c: 81.148Å
α: 90° β: 100.77° γ: 90°
R-values:
R R work R free
0.259 0.257 0.307
Expression system: Escherichia coli

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