4pei

X-ray diffraction
1.95Å resolution

Dbr1 in complex with synthetic branched RNA analog

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Metallophos domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 356 amino acids
Theoretical weight: 41.2 KDa
Source organism: Entamoeba histolytica
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: C4M1P9 (Residues: 1-354; Coverage: 100%)
Gene name: EHI_062730
Sequence domains: Calcineurin-like phosphoesterase
RNA (5'-R(*UP*AP*AP*CP*A)-3') Chains: V, W, X, Y, Z
Molecule details ›
Chains: V, W, X, Y, Z
Length: 5 nucleotides
Theoretical weight: 1.55 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*(G46)P*U)-3') Chains: Q, R, S, T, U
Molecule details ›
Chains: Q, R, S, T, U
Length: 2 nucleotides
Theoretical weight: 622 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 73.037Å b: 142.479Å c: 214.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.229
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided