› 4pcu

X-ray diffraction
3.58Å resolution

Crystal structure of delta516-525 E201S human cystathionine beta-synthase with AdoMet

Released:
DOI: 10.2210/pdb4pcu/pdb
PDB 4pcu coloured by chain and viewed from the front.
PDB 4pcu coloured by chain and viewed from the side.
PDB 4pcu coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural insight into the molecular mechanism of allosteric activation of human cystathionine ��-synthase by S-adenosylmethionine.
Ereño-Orbea J, Majtan T, Oyenarte I, Kraus JP, Martínez-Cruz LA
Proc. Natl. Acad. Sci. U.S.A. 111 E3845-52 (2014)
PMID: 25197074

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 60.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35520 (Residues: 1-551; Coverage: 98%)
Gene name: CBS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP

Cofactor: Ligand SAM 2 x SAM

Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P3121
Unit cell:
a: 141.354Å b: 141.354Å c: 108.528Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.257 0.277
Expression system: Escherichia coli

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Citations

15 review citations

Harnessing the Benefits of Endogenous Hydrogen Sulfide to Reduce Cardiovascular Disease.
Casin et al. (2021)
14 more

11 mentions without citation

Structural Basis for Allostery in PLP-dependent Enzymes.
Tran et al. (2022)
10 more