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Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces.

Chen R, Qi J, Yuan H, Wu Y, Hu W, Xia C
J Struct Biol 189 259-68 (2015)
Cited: 7 times
EuropePMC logo PMID: 25592778

Abstract

Short-chain pentraxins (PTXs), including CRP and SAP, are innate pattern recognition receptors that play vital roles in the recognition and elimination of various pathogenic bacteria by triggering the classical complement pathway through C1q. Similar to antibodies, pentraxins can also activate opsonisation and phagocytosis by interacting with Fc receptors (FcRs). Various structural studies on human PTXs have been performed, but there are no reports about the crystal structure of bony fish pentraxins. Here, the crystal structures of zebrafish PTX (Dare-PTX-Ca and Dare-PTX) are presented. Both Dare-PTX-Ca and Dare-PTX are cyclic trimers, which are new forms of crystallised pentraxins. The structures reveal that the ligand-binding pocket (LBP) in the recognition face of Dare-PTX is deep and narrow. Homology modelling shows that LBPs from different Dare-PTX loci differ in shape, reflecting their specific recognition abilities. Furthermore, in comparison with the structure of hCPR, a new C1q binding mode was identified in Dare-PTX. In addition, the FcR-binding sites of hSAP are partially conserved in Dare-PTX. These results will shed light on the understanding of a primitive PTX in bony fish, which evolved approximately 450 million years ago.

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  1. Hydroxycholesterol binds and enhances the anti-viral activities of zebrafish monomeric c-reactive protein isoforms. Bello-Perez M, Falco A, Novoa B, Perez L, Coll J. PLoS One 14 e0201509 (2019)


Reviews citing this publication (1)

  1. Regulation of C-reactive protein conformation in inflammation. Yao Z, Zhang Y, Wu H. Inflamm Res 68 815-823 (2019)

Articles citing this publication (5)

  1. Collagen-like proteins (ClpA, ClpB, ClpC, and ClpD) are required for biofilm formation and adhesion to plant roots by Bacillus amyloliquefaciens FZB42. Zhao X, Wang Y, Shang Q, Li Y, Hao H, Zhang Y, Guo Z, Yang G, Xie Z, Wang R. PLoS One 10 e0117414 (2015)
  2. In silico Analysis Revealed High-risk Single Nucleotide Polymorphisms in Human Pentraxin-3 Gene and their Impact on Innate Immune Response against Microbial Pathogens. Thakur R, Shankar J. Front Microbiol 7 192 (2016)
  3. Modulation of the Tissue Expression Pattern of Zebrafish CRP-Like Molecules Suggests a Relevant Antiviral Role in Fish Skin. Bello-Perez M, Adamek M, Coll J, Figueras A, Novoa B, Falco A. Biology (Basel) 10 78 (2021)
  4. Secretory quality control constrains functional selection-associated protein structure innovation. Cheng B, Lv JM, Liang YL, Zhu L, Huang XP, Li HY, Potempa LA, Ji SR, Wu Y. Commun Biol 5 268 (2022)
  5. Characterization of the innate immune response to Streptococcus pneumoniae infection in zebrafish. Saralahti AK, Harjula SE, Rantapero T, Uusi-Mäkelä MIE, Kaasinen M, Junno M, Piippo H, Nykter M, Lohi O, Rounioja S, Parikka M, Rämet M. PLoS Genet 19 e1010586 (2023)


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