› 4p6k

X-ray diffraction
2.7Å resolution

Crystal Structure of the Computationally Designed Transmembrane Metallotransporter with 4-bromophenylalanine in Lipidic Cubic Phase

Released:
DOI: 10.2210/pdb4p6k/pdb
PDB entry 4p6k coloured by chain, front view.
PDB entry 4p6k coloured by chain, side view.
PDB entry 4p6k coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
De novo design of a transmembrane Zn²⁺-transporting four-helix bundle.
Joh NH, Wang T, Bhate MP, Acharya R, Wu Y, Grabe M, Hong M, Grigoryan G, DeGrado WF
Science 346 1520-4 (2014)
PMID: 25525248

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Computationally Designed Transporter of Zn(II) and Proton Chain: A
Molecule details ›
Chain: A
Length: 26 amino acids
Theoretical weight: 3.02 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I4122
Unit cell:
a: 46.87Å b: 46.87Å c: 60.603Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.296 0.295 0.304
Expression system: Not provided

Quick links

Citations

47 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
46 more

3 mentions without citation

General lack of structural characterization of chemically synthesized long peptides.
Boutin et al. (2019)
2 more